FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_hw2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_hw2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17627512
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAT220100.016.5680269
GCGAATC145450.016.3188869
AATCTCG89150.015.01502469
AGGCGAA292000.014.39063869
TAAGGCG435300.012.56192669
AAGGCGA378400.012.43594769
ACTAAGG666150.012.15504969
CTAAGGC555050.011.9961269
GACTAAG782250.011.56826869
TAGACCG31250.011.4820955
GCGAATG50200.011.40827669
GAATCTC130050.011.38053269
CGAATCT138700.011.01901868
GTATAGG38950.010.6290781
AGACTAA935100.010.24919669
GTATTAG49850.010.1735841
GTATAGA56650.010.1704081
TAGACAG41850.09.9753415
ATCTCGT64250.09.93377369
CCTAGAC32550.09.8575123
Produced by FastQC (version 0.11.2)