FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_hw1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_hw1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17687751
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAG12250.020.559847
CCCGGGG8900.018.2192731
GCGAATC97350.018.1802469
TCACACG62800.018.07487929
GGCGAAT173200.017.32964169
CCCATGC75050.017.239018
CCATGCC80150.016.7014359
AGGCGAA213400.015.89195569
CTAGCCG15800.015.5038353
AGTTCCG34850.015.444045
CACACGT74700.015.1028530
CACGCCC31300.014.21874118
CGGCCAG32050.014.2091811
ATCCCAT106100.013.9828556
CTAGTAG40700.013.648013
TGCACGC32650.013.6308316
ACACGTT86800.013.59371431
CGCGAGG17650.013.4876448
GTCTAGT42600.013.4437881
GTATTAG40900.013.41210651
Produced by FastQC (version 0.11.2)