FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_hw0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_hw0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15222800
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC42200.015.4521318
TCACACG32550.014.62735229
CCATGCC48650.014.5377989
GTATATA46200.014.2632211
CGCGTGA12800.012.39888427
GTATAGA42700.012.2812321
CCGCGAG5950.012.1771117
GTATTAG40900.011.80948451
CGCGTAG15650.011.68351320
CACACGT42350.011.40522130
GTATAAA62150.011.3799121
CGCGCAT15950.011.2473940
TATACTG47950.011.0090285
ACCCGCG11600.011.00452633
GACGCGC14550.010.90696538
TATTATA77100.010.8740042
CCCGCGC11200.010.78146334
GTATTAT87850.010.7212921
GTCTAGT36750.010.7022161
GTATAGG41250.010.5383541
Produced by FastQC (version 0.11.2)