FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_hq4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_hq4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13283056
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGGG14300.018.33673727
TCACACG39600.017.86116229
CCCATGC49500.017.77358
CCCGGGG3900.017.6924111
CCATGCC54250.016.5984429
AGTTCCG25700.016.1098925
CACACGT48900.014.67504330
GTCTAGT36250.014.6566371
GACGGGA17000.014.61262128
CGGGTCG8300.014.13218818
CGGCCAG20450.013.6651161
GTATATA36700.013.4428581
CGTACTG38050.013.41997054
ATACGGG36150.013.07437238
TACGGGA34950.013.02972639
CAAACGG63950.012.94739815
CACGCCC21100.012.75324518
GATCGTA38850.012.69891551
TCCCATG74250.012.2671557
ATCCCAT87100.012.2005316
Produced by FastQC (version 0.11.2)