FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_hq3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_hq3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16794766
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCTA15950.019.68332310
CCCATGC57800.016.7132958
GTATTAG29950.016.7030331
GTATATA22750.016.6815151
GTATAGA35600.016.4749131
CTCCTAA27450.016.2133051
GACGCGC22800.015.88782238
CCCGGGG4450.015.5057991
TCGTTCG26800.015.44766521
TCACACG43300.015.37841829
ATCCCCC17550.015.13652215
GTATTAA31750.015.1041141
CCATGCC66150.015.0204949
CGCGCAT23700.014.99335540
GGTCTCG26550.014.9434117
GTATAAC36450.014.5763011
ACGCGCA25750.014.33561939
GTATTAT78650.014.2563641
TATATAC19250.014.1591123
TATACTG35050.014.0763155
Produced by FastQC (version 0.11.2)