FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_hq2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_hq2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13829894
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAG11150.023.8260427
GACTCCC16850.021.29329143
CGCTAGG10450.020.13830446
CTCCCGT18900.019.89637845
CGCGAGG14150.019.0182028
GCGGGTG11100.018.6496456
CCCGGGG5100.018.2650221
CTAGCCG14700.017.8376243
CTCGGCC14850.017.65750727
CGTACTG43500.017.131894
ACTCCCG21950.017.13173544
CCCGTGG21550.017.12954547
GATCGTA43300.016.9714261
CGGGTGA12600.016.7030327
ATCGTAC43000.016.4483172
CCTCGGC15750.016.42950826
ACGCTAG23500.016.14985726
CACGCCC23850.016.05635818
GGACGGG14100.015.66041227
CCCATGC59050.015.4830788
Produced by FastQC (version 0.11.2)