FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_hq1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_hq1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16053360
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAG12450.033.8085487
CTCGGCC15950.025.09251427
CTAGCCG17350.024.4592443
CGCGAGG17450.024.1211368
GCCGCGA18950.022.2123646
CACGCCC32650.020.81587618
CGCACCG17800.020.54452951
AGCCGCG20650.020.3836155
CGGCCAG34450.019.9290391
TGCACGC34850.019.00690816
CCTCGGC21700.018.92056526
CGTGCCC19500.018.75346856
GCACGCC36750.018.30586617
CACGGAT37400.017.8036169
ACGCCCT38800.017.783219
AGTTCCG31050.017.556465
CCCATGC71150.017.3596698
GCCAGAC40400.017.1653273
TCACACG59100.016.7549329
TAGCCGC25650.016.6791174
Produced by FastQC (version 0.11.2)