FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_hq0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_hq0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15229971
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAG9350.022.878017
CTAGCCG12400.017.528653
GACTCCC15500.017.13827943
CACGCCC22500.017.01990918
CGCTAGG12700.016.84209646
CCCGGGG5200.016.5863421
CCCATGC53550.016.4292378
CGCGAGG13250.016.404448
CTCCCGT17650.015.44153945
CGGTGCG13450.015.3906069
TGCACGC24650.015.25554716
CCATGCC59450.015.2046419
GCACGCC24800.015.16332417
AGTTCCG28450.015.1587875
CGCACCG13050.015.06858751
GCGAATC35500.014.86883669
CGGCCAG28150.014.7067531
GGACGGG14050.014.4883727
ACGCCCT26100.014.40787319
CACGGAT28350.014.116659
Produced by FastQC (version 0.11.2)