FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_ew4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_ew4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14827585
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC88550.019.98828
CCATGCC92600.019.44849
TCACACG79100.019.23552529
CAAACGG96700.016.58972215
CACACGT94450.016.43735730
CACGTTC96450.016.34684632
GTCTAGT56800.015.913981
TGGGCCG27950.015.79967433
AGTTCCG44950.015.6586965
ACACGTT100850.015.56527931
AAATCCC106250.015.5545964
TTCACAC101650.015.5452828
GGGCCGT28100.015.34700534
TCCCATG119700.014.988467
CCAAACG108650.014.89214414
ATCCCAT126350.014.746036
GGCCGTA29500.014.50172335
AATCCCA122400.014.4889475
TAAATCC119700.014.4121263
ACGTTCT112350.014.06411433
Produced by FastQC (version 0.11.2)