FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_ew3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_ew3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14190294
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC87950.019.928048
CCATGCC92550.019.5333279
TCACACG76400.018.65095329
CAAACGG92900.017.08267415
AAATCCC98550.016.69964
AGTTCCG40950.015.9240595
ATCCCAT116600.015.6827856
CACACGT92500.015.55332930
TCCCATG114250.015.521917
CACGTTC96750.015.47632432
GTCTAGT49050.015.403751
CCAAACG105800.015.32589714
AATCCCA115650.015.1552795
TAAATCC113350.015.0669893
ACACGTT98850.014.79851731
TTCACAC102550.014.46675228
CATGCCA125650.014.25017410
GTTAAAT128800.014.1697191
CCCGGGG9300.014.09684851
CGTACTG49000.014.0824514
Produced by FastQC (version 0.11.2)