FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_ew2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_ew2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15495393
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC99250.019.5359388
CCATGCC103100.019.2077949
TCACACG89800.018.48081429
GTCTAGT52150.016.8035431
AGTTCCG47450.016.2146785
CAAACGG110700.016.0811315
AAATCCC116400.016.0353984
CACACGT103900.015.87223330
TCCCATG124850.015.7236597
CACGTTC112100.015.41900632
CCAAACG117150.015.37238614
TAAATCC127500.015.2888143
ATCCCAT130350.015.0340856
AATCCCA131350.014.8408255
TTCACAC117050.014.67937128
ACACGTT114850.014.65934431
CCCGGGG12850.014.4981341
GTTAAAT137900.014.4105231
CATGCCA134150.014.01601510
ACGTTCT124700.013.77803233
Produced by FastQC (version 0.11.2)