FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_ew1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_ew1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11480675
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC66200.020.116988
CCATGCC71550.019.3836989
TCACACG59200.018.99918729
CCCGGGG7200.017.249971
AGTTCCG33200.016.6272745
AAATCCC79200.016.335984
CAAACGG75350.016.20833215
GTCTAGT41100.016.20071
CACACGT72350.015.78386930
TCCCATG89000.015.4673667
CCAAACG78600.015.40639514
ATCCCAT92700.015.3340416
CACGTTC78250.015.03467432
CGTACTG41600.014.9285734
ACACGTT80950.014.74630631
TAAATCC96200.014.4892353
GATCGTA43500.014.3551471
ATCGTAC42400.014.239922
TTCACAC82700.014.18445228
AATCCCA94650.014.1432845
Produced by FastQC (version 0.11.2)