FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_ew0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_ew0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11223735
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC55950.020.1022828
TCACACG49050.018.99205429
CCATGCC60800.018.441829
TCCCATG77750.015.3089237
CAAACGG66500.015.25266815
CACACGT61750.015.19730
AAATCCC71500.014.476014
CACGTTC67750.014.41129632
ATCCCAT81600.014.37554556
ACACGTT68550.014.04179631
CCAAACG71500.013.9930414
TTCACAC72050.013.79131628
TAAATCC79750.013.3245843
GTTAAAT89650.013.0842471
ACGTTCT76200.013.03951633
AATCCCA85500.012.6706565
CATGCCA88600.012.42158610
ATCGTAC34850.012.3747662
GTCTAGT35600.012.2107031
CCTAAGC15900.012.1513513
Produced by FastQC (version 0.11.2)