FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_eq4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_eq4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12432472
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC59600.016.382538
TCACACG49600.016.13803329
TAGCCTA27100.015.78600510
CCATGCC64350.015.7622639
GTATAGG31750.015.4298231
CGCGTGA24500.014.64551227
GCGCGTG25500.014.61237626
GTCTAGT47950.014.2459981
ATGCGCG25550.014.04364124
AGTTCCG34000.014.0037545
GACAGGC85750.013.96089969
TGCGCGT27100.013.87696525
GTATTAG30050.013.7769491
GTATATA53650.013.6970221
CCCGGGG5600.013.5534841
GATCGTA42500.013.4752081
GTATAAG35650.013.4515271
TATACTG46950.013.4480645
CTCCTAA38500.013.2622531
CAAACGG65600.013.20047915
Produced by FastQC (version 0.11.2)