FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_eq3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_eq3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16219556
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC83650.019.5911718
CCATGCC91450.018.6366569
TCACACG73650.017.94172329
CAAACGG92800.016.1346115
ATCCCAT113150.015.1852146
GTCTAGT54250.015.1357261
AAATCCC101450.014.7598224
TCCCATG112850.014.7360437
CACACGT90850.014.50656130
GTATATA58050.014.3232271
TTCACAC95550.014.19044328
CACGTTC97900.014.09624232
CCAAACG108200.014.02958414
AATCCCA113650.013.90407855
GTTAAAT120450.013.8918791
TAAATCC112300.013.8253543
AGTTCCG43850.013.6120235
ATGCGCG37050.013.59565324
GACAGGC210900.013.43022469
ACACGTT104950.013.37944331
Produced by FastQC (version 0.11.2)