FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_eq2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_eq2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16489010
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACACG84650.021.31679229
CCATGCC99150.021.2608099
CCCATGC96650.021.240128
AAATCCC106850.018.1150444
CAAACGG105850.018.08923715
CACACGT102400.017.68848430
ATCCCAT125200.017.5545676
TCCCATG124900.017.3479547
AATCCCA121100.016.8382515
CACGTTC114600.016.798832
TTCACAC110600.016.78287328
CCAAACG116100.016.67050614
ACACGTT117500.016.35492731
AGTTCCG42800.016.0421665
GTTAAAT132100.015.931271
TAAATCC124700.015.7709613
TAGCCTA32950.015.60092310
CCCGGGG12450.015.5182271
ACGTTCT132900.014.61544533
GTATATA50300.014.60948851
Produced by FastQC (version 0.11.2)