FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_eq1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_eq1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15045599
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC84600.019.2087088
CCATGCC88950.018.268669
TCACACG71850.018.24712229
AGTTCCG41750.016.197745
GATCGTA52000.015.2598071
CAAACGG95300.015.05979915
AAATCCC97900.015.0133934
GTCTAGT53500.014.9609351
CGTACTG56200.014.4272494
CACACGT91650.014.41729130
TCCCATG118450.014.2729737
ATCCCAT120800.014.0241536
CACGTTC97450.013.98403732
TTCACAC98300.013.93400428
GTTAAAT118000.013.8586481
ACACGTT97550.013.82823831
GCCCGGG10100.013.6644477
CCCGGGG10150.013.5969528
ATCGTAC56850.013.5944252
CCAAACG107550.013.56893614
Produced by FastQC (version 0.11.2)