FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_eq0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_eq0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14356719
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGGG9500.019.6108191
AGTTCCG41350.019.6078535
TCACACG76350.018.43700229
CCCATGC89250.018.1686558
GTCTAGT55200.017.9377691
CCATGCC92800.017.547929
GGGCCGT22000.015.99547234
GTTCCGA50850.015.8091016
CAAACGG96050.015.44493515
TAGTTCC57550.015.4072614
CCGGGGA12400.015.3028612
AAATCCC98000.015.2792254
GATCGTA54750.015.1865291
CACACGT95900.014.85773130
CACGTTC98750.014.81323132
TCCCATG118750.014.4106717
ACGGCCC23050.014.36897810
CGTACTG59150.014.3488824
ACACGTT103900.014.34462631
ATCGTAC57550.014.3279552
Produced by FastQC (version 0.11.2)