FastQCFastQC Report
Fri 6 Dec 2024
HLF5YDRX5_l02_n01_5-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLF5YDRX5_l02_n01_5-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6683845
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCTTAAATCTCGTAT1301291.9469182783263226TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCTTAAATCGCGTAT545290.8158328028253198TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCTTAAATCTCGGAT133640.19994479225655293TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCTTAAATCTCGTTT122930.18392108135362206TruSeq Adapter, Index 23 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94960.14207391104970268No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)