FastQCFastQC Report
Fri 6 Dec 2024
HLF5YDRX5_l02_n01_11-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLF5YDRX5_l02_n01_11-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402202
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590574.211732689013424No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA168821.203963480297418No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCGTATCTCGTAT106720.7610886305967328TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCGTATCGCGTAT45380.3236338273658146TruSeq Adapter, Index 1 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)