FastQCFastQC Report
Fri 6 Dec 2024
HLF5YDRX5_l01_n02_LA_3R-exp1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLF5YDRX5_l01_n02_LA_3R-exp1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10289725
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT458480.4455707028127574No Hit
GTCATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAA407180.39571514301888533No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA255400.24820877137144093No Hit
ATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAATTATATG248620.2416196739951748No Hit
ATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAATTA205470.19968463685861382No Hit
AAATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTAATTCA166880.16218120503706368No Hit
TTTTTAAATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTA158110.1536581395518345No Hit
CATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAATT150950.14669974173265077No Hit
ATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAAC138780.13487240912658016No Hit
GCATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAATTATA124060.12056687617987846No Hit
ATCCAATTCCCGATCGTTCAAACATTTGGCAATAAAGTTTCTTAAGATTG111810.108661796112141No Hit
CCCGATCGTTCAAACATTTGGCAATAAAGTTTCTTAAGATTGAATCCTGT106830.10382201662337916No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)