FastQCFastQC Report
Fri 6 Dec 2024
HLF5YDRX5_l01_n02_LA_2R-exp1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLF5YDRX5_l01_n02_LA_2R-exp1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9026666
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT784480.8690694881144378No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA699840.7753028637594435No Hit
GTCATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAA293320.3249483253285322No Hit
ATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAATTATATG204310.22634048938999182No Hit
TTTTTAAATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTA147770.1637038525630615No Hit
ATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAATTA138960.15394388138433393No Hit
AAATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTAATTCA129160.14308715975532937No Hit
ATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAAC116830.12942763142006142No Hit
CATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAATT105740.1171418107194838No Hit
TTTTAAATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTAA94150.10430207565007944No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)