FastQCFastQC Report
Fri 6 Dec 2024
HLF5YDRX5_l01_n01_LA_3R-exp1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLF5YDRX5_l01_n01_LA_3R-exp1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10289725
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT495650.4816941171897208No Hit
GTCATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAA436700.42440395637395556No Hit
ATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAATTATATG265370.257898048781673No Hit
ATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAATTA208460.2025904482384126No Hit
AAATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTAATTCA166230.16154950691102046No Hit
TTTTTAAATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTA163820.1592073646283064No Hit
CATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAATT153640.14931400013119886No Hit
ATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAAC146860.14272490275493271No Hit
GCATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAATTATA124860.12134435079654705No Hit
ATCCAATTCCCGATCGTTCAAACATTTGGCAATAAAGTTTCTTAAGATTG106700.1036956769981705No Hit
GTTAATTATTACATGCTTAACGTAATTCAACAGAAATTATATGATAATCA105930.102947357679627No Hit
CCCGATCGTTCAAACATTTGGCAATAAAGTTTCTTAAGATTGAATCCTGT104370.1014312821771233No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)