FastQCFastQC Report
Fri 6 Dec 2024
HLF5YDRX5_l01_n01_LA_2R-exp1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLF5YDRX5_l01_n01_LA_2R-exp1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9026666
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT934471.0352327204750902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530960.5882127465445159No Hit
GTCATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAA308260.3414992866690758No Hit
ATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAATTATATG212560.2354800764756334No Hit
TTTTTAAATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTA154690.1713700274276239No Hit
ATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAATTA142610.1579874562767693No Hit
AAATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTAATTCA133030.14737445697004853No Hit
ATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAAC117540.1302141898237954No Hit
CATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAATT114210.12652512012740916No Hit
TTTTAAATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTAA95770.10609675820507816No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)