FastQCFastQC Report
Fri 6 Dec 2024
HLF5YDRX5_l01_n01_LA_22R-exp2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLF5YDRX5_l01_n01_LA_22R-exp2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9181
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCTTTCCCTACACGA1331.4486439385687835TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACTTACAAAACAAAA370.40300620847402246TruSeq Adapter, Index 5 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACACTTTCCCTACACGA290.3158697309661257TruSeq Adapter, Index 5 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCTTACCCTACACGA250.2723014922121773TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACACATTCCCTACACGA210.22873325345822895TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCATTCCCTACACGA210.22873325345822895TruSeq Adapter, Index 25 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCTTTCCCAACACGA170.18516501470428057TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACACTTACCCAACACGA160.17427295501579348TruSeq Adapter, Index 5 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCATACAAAACAAAA160.17427295501579348TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACACTTTCCCAACACGA160.17427295501579348TruSeq Adapter, Index 5 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACACTTACCCTACACGA150.16338089532730637TruSeq Adapter, Index 5 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCATACCCTACACGA140.15248883563881932TruSeq Adapter, Index 25 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCATTCCCAACACGA130.1415967759503322TruSeq Adapter, Index 25 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATATTACAAAACAAA120.13070471626184513TruSeq Adapter, Index 8 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.13070471626184513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCATACCCAACACGA120.13070471626184513TruSeq Adapter, Index 25 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACACTATCCCTACACGA110.11981265657335803TruSeq Adapter, Index 5 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACACAAACCCTACACGA100.10892059688487093TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGACACACACATTCCCTACACGA100.10892059688487093TruSeq Adapter, Index 5 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGACACACACATACCCTACACGA100.10892059688487093TruSeq Adapter, Index 5 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)