FastQCFastQC Report
Fri 6 Dec 2024
HLF5YDRX5_l01_n01_LA_1R-exp1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLF5YDRX5_l01_n01_LA_1R-exp1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9531764
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAA369510.3876617171805764No Hit
ATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAATTATATG208880.21914096907980515No Hit
ATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAATTA193030.20251235762866138No Hit
TTTTTAAATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTA177040.1857368688523971No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171690.18012405678529178No Hit
ATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAAC163300.17132190851556964No Hit
AAATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTAATTCA151830.15928845909319617No Hit
CATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAATT142680.1496889767728198No Hit
GCATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAATTATA107410.11268638207995918No Hit
TTTTAAATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTAA100650.10559430552414012No Hit
CCCGATCGTTCAAACATTTGGCAATAAAGTTTCTTAAGATTGAATCCTGT96650.10139781052069691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)