FastQCFastQC Report
Fri 6 Dec 2024
HLF5YDRX5_l01_n01_9-R-KL.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLF5YDRX5_l01_n01_9-R-KL.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150164
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCGGTT28401.8912655496656987TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCGTTT26271.7494206334407716TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCGTAT16671.1101196025678592TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCGGAT15681.04419168375909TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCGCGGTT12040.8017900428864442TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCGCGTTT9990.6652726352521243TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCGCGTAT5690.3789190485069657TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCGGGT5670.3775871713593138TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCGCGGAT5210.34695399696332013TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCGTGT3980.26504355238272825TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCGCGGGT3190.21243440505047817TruSeq Adapter, Index 18 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2060.13718334620814576No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCGCGTGT1910.1271942676007565TruSeq Adapter, Index 18 (97% over 37bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT1780.11853706614101914Illumina Single End PCR Primer 1 (96% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)