FastQCFastQC Report
Fri 6 Dec 2024
HLF5YDRX5_l01_n01_3-C-KL.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLF5YDRX5_l01_n01_3-C-KL.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7218698
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGATTCATCTCGTAT4419096.121727214519848TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGATTCATCGCGTAT2360863.270478970030329TruSeq Adapter, Index 10 (97% over 36bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT259910.3600510784631799Illumina Single End PCR Primer 1 (96% over 30bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGATTCATCTCGTA84250.11671079743189146TruSeq Adapter, Index 3 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)