FastQCFastQC Report
Fri 6 Dec 2024
HLF5YDRX5_l01_n01_19-C-KL.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLF5YDRX5_l01_n01_19-C-KL.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11729284
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACATCTCGTAT189650.16168932391781118TruSeq Adapter, Index 20 (97% over 37bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT169440.14445894566113326No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT144580.12326413104158788No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACATCGCGTAT127830.1089836344656673TruSeq Adapter, Index 20 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)