FastQCFastQC Report
Fri 6 Dec 2024
HLF5YDRX5_l01_n01_17-R-KL.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLF5YDRX5_l01_n01_17-R-KL.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3246869
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT872252.686434223247073No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGGTTATCTCGTAT538031.6570733220219231TruSeq Adapter, Index 9 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360381.1099308287460936No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGGTTATCGCGTAT235840.7263613037667981TruSeq Adapter, Index 9 (97% over 37bp)
AAAGTTCCCAGCGTCTCATCGAGCTAGTGTACGTCGCGGTGCCGAAAGAA40780.12559792218287832No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT36120.11124563387066123Illumina Single End PCR Primer 1 (96% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)