FastQCFastQC Report
Fri 6 Dec 2024
HLF5YDRX5_l01_n01_16-R-KL.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLF5YDRX5_l01_n01_16-R-KL.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12040374
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTAAGCATCTCGTAT678110.5631967910631347TruSeq Adapter, Index 11 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTAAGCATCGCGTAT324270.26931887663954623TruSeq Adapter, Index 11 (97% over 38bp)

[OK]Adapter Content

Adapter graph