FastQCFastQC Report
Fri 6 Dec 2024
HLF5YDRX5_l01_n01_15-R-KL.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLF5YDRX5_l01_n01_15-R-KL.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8338439
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAAGCAATCTCGTAT861311.0329391388484104TruSeq Adapter, Index 10 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT441590.5295835347599233No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAAGCAATCGCGTAT322560.38683499393591536TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAAGCAATCTCGTTT254670.3054168771876847TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAAGCAATCTCGGAT203190.24367870293228747TruSeq Adapter, Index 10 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA198420.23795820776526638No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAAGCAATCGCGTTT103520.12414793704193315TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)