FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10604049
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN370390.34929110568991145No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG169430.15977859023473015No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA123000.11599342854790656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA15950.089.1144941
GCAGGTA35200.066.084159
CGAAAGC31250.063.820833
GAGCAAA128400.061.2233661
GCAAAAG157000.049.8888973
AGCAAAA158050.049.464752
GCAGGGT85650.044.588029
CAAAAGC197250.039.782314
GGTCGTT6200.036.237197
GAAAGCA57000.036.13464
GGTAGTT18100.035.236167
TCTACAC23250.034.3122753
CAATTCG7800.033.4458969
AAAGCAG279800.033.395886
CTACACT23950.032.703794
AGCAGGG158650.032.4789248
AAGGGTG52500.032.4618955
AACAAGG87250.032.333722
ACAAGGT27550.032.115753
GTTACGT2051.1377779E-831.837691
Produced by FastQC (version 0.11.5)