FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n02_TR_PLASMID_CONTROL_P2_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n02_TR_PLASMID_CONTROL_P2_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012459
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGGTACA29250.2889005875793489No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG26840.26509715455144356No Hit
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGGATGATGATGGGCAT26330.26005991353723956No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG25550.2523558978684569No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG25040.24731865685425286No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG23380.23092293120017698No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTTT22660.22381153212130073No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT21710.2144284361144501No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC21570.21304566407133524No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA18440.18213083196455362No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA18150.1792665184466729No Hit
CCTATATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGG17920.17699482151869853No Hit
ATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGGCTGTT16910.16701910892194155No Hit
ACATATAGGTGCCATAGAGGAGACACACAAATTCAGACGAGAAGATCATT16650.164451103699014No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT16390.16188309847608642No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG15960.15763601291509088No Hit
GATATACATATAGGTGCCATAGAGGAGACACACAAATTCAGACGAGAAGA15940.15743847405178876No Hit
GAATAAGAGAGGCTATCTAATAAGAGCTTTGACATTGAACACGATGACCA15790.15595693257702287No Hit
CTCTTATTCACTCTTTGTTTTTTCTTCCCTATTGTTCTTTGTGTGACCAT15730.1553643159871165No Hit
GTATAAGTTTGGTCCCCCATCTGATACCAATAGTCCTGCCCTTGATTGGG15590.15398154394400168No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAACCTTTAGGAA15490.1529938496274911No Hit
TCATATAGGAGACCGATTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC15190.1500307666779593No Hit
ATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAATTTGCAGGTCCT14510.14331444532568727No Hit
TCCTTGAAGAATCCCACCCAGGTATCTTTGAGAACTCATGCCTTGAAACA14360.14183290385092137No Hit
GGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTC14170.13995628464955123No Hit
ATGTAATCATCGCCAAAAACATTCGAGGGTTTTGATTTTCATTCCACTTA13950.13778335715322793No Hit
GGCTAATAGATTTCCTCAAGGATGTGATGGAATCAATGGATAAAGAGGAA13870.13699320170001947No Hit
GTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGT13770.13600550738350886No Hit
GTTTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAATTT13420.13254857727572178No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT13250.1308694969376538No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC13230.13067195807435164No Hit
TCCCTGAAGTCTGCTTAAAGTGGGAGCTAATGGATGAGAATTATCAGGGA13140.1297830331894921No Hit
CTATTAGCCTTCCTGATTCATTAGCTGTTAGTCCATTCGATCTAAAAACT12830.12672118080830927No Hit
AAATAAAACAGGGACATTTGAATTCACAAGCTTTTTTTATCGATATGGAT12720.12563471706014762No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT12580.1242519450170328No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT12330.1217827092257563No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT12320.12168393979410524No Hit
TTCTTAATCCGTCCAGACTCGAAGTCAATTCTGGCATCAATCCGGGCCCT11980.11832577911796921No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG11840.11694300707485439No Hit
GTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATG11770.11625162105329696No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC11640.1149676184418332No Hit
GATTACATATATCACAAAAAATCAACCTGAGTGGTTCAGAAACATCCTGA11470.11328853810376519No Hit
GGCTAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTCCAATCATAAG11270.111313149470744No Hit
CCATAGAAGTTTTTAGATCGAATGGACTAACAGCTAATGAATCAGGAAGG11140.11002914685928024No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT11130.10993037742762918No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG11030.10894268311111859No Hit
GTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATA11020.10884391367946751No Hit
GTCCTATATAAATAAAACAGGGACATTTGAATTCACAAGCTTTTTTTATC10960.10825129708956116No Hit
CATAGTGAATGCACCAAATCATGAGGGAATACAAGCAGGAGTGGATAGAT10940.10805375822625905No Hit
AATAAGAGCTTTGACATTGAACACGATGACCAAAGATGCAGAGAGAGGTA10920.10785621936295692No Hit
ATTCTAGACGGGCCGGCCCCTGCGTGTGGCACGGGCGGCCGGGAGGGCGT10920.10785621936295692No Hit
TTATTGGTGCGATGCTCAGGATGTTTCTGAACCACTCAGGTTGATTTTTT10920.10785621936295692No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC10900.1076586804996548No Hit
GCATTAGCCAACACCATAGAAGTTTTTAGATCGAATGGACTAACAGCTAA10830.10696729447809737No Hit
ATCCAGGAGTGTGTAGTTGCAACGGCATCATATTCCATACTTTTGGCTGG10650.10518944470837832No Hit
ATATGTAATCATCGCCAAAAACATTCGAGGGTTTTGATTTTCATTCCACT10630.10499190584507619No Hit
ATTCCAGACACTCCAAAACTGGGAAGCTCCATGCTAAAATTAGCCACAAA10580.1044980586868209No Hit
AACTTATACAATATCCGGAACCTTCACATCCCTGAAGTCTGCTTAAAGTG10520.10390544209691453No Hit
GGGTACACCATGGACACAGTCAACAGAACACACCAATATTCAGAGAAGGG10490.10360913380196136No Hit
GTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGGCTGTTGC10390.10262143948545077No Hit
CCATATATGGGCTATGAACTAATGACCCCGTAATTGATTACTATTAATAA10340.10212759232719547No Hit
GCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTG10280.10153497573728912No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATATTT700.093.20441145
TCCTCCC608.2945917E-1084.57437145
ACCGGCC353.4391036E-582.84836145
ACATCTT606.8099307E-660.410267145
CGACGAA1550.056.123085145
GGAAGCC400.00605344454.369236145
AGGCCCC553.2207047E-452.721684145
CCGTTCC450.00964320548.328213145
CGGTGGG1351.0270014E-842.95841145
CACGCCC1201.9751133E-742.287186145
TGTCTTT700.00105719841.42418145
ACTGATG9000.040.2754978
TCTCGGG907.487179E-540.27351145
AAACCCC1105.268197E-639.541264145
TACTGAT9050.039.2480437
TATACTG19450.038.3854525
TTATACT10200.038.3725934
AACTCCC951.0295416E-438.15385145
CTGATGA9400.037.7885449
CATATAC9950.036.42113
Produced by FastQC (version 0.11.5)