FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n02_TR_564-2_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n02_TR_564-2_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011407
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA15750.078.697471
GCGAAAG16300.076.490532
GAGCGAA5850.073.1040341
CGAAAGC17400.072.488113
GAGCAAA38650.043.6971
TATACGA1752.4374458E-937.2786757
CAAAAGC48200.034.1394424
GCAAAAG50550.033.268533
GGTTACG1352.1464055E-532.2153361
AGCAAAA53500.031.2986092
TACGAAC2101.4444595E-831.0647939
GAAAGCA57650.022.8849374
AAAGCAG93450.022.3392816
ACCCGAC1700.003097257321.3195067
AAAAGCA78200.021.135175
GTATAGG9150.018.220151
GTATATA19600.017.7513081
GTATAAG13600.017.5881541
AAGCAGG118000.017.384437
ATATACG4358.6072214E-716.663526
Produced by FastQC (version 0.11.5)