FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n02_TR_563-5_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n02_TR_563-5_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427852
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG25150.17613870345105795No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC21130.14798452500679343No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA21100.14777441919750786No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG20370.14266184450489267No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA18660.13068581337561594No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG18570.13005549594775928No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG15810.11072576149348812No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA15790.11058569095396442No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA14930.10456265775444513No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5200.0111.5212861
TATACCG203.870386E-4108.7218255
AGCGAAA10050.064.194091
CGAAAGC10700.060.288113
GCGAAAG10950.058.9178662
GAGCAAA41900.053.631361
GTTACGT1650.052.7191541
ATCTCGC1351.9099389E-1048.325891
GCGACTA1850.047.0164874
GTTATGT9600.045.3055231
GCAGGTA11000.044.150749
AAGCAGG64650.042.827267
TACACGG1252.7233182E-740.5937462
CAAAAGC56900.038.4711654
TATGTGT11300.037.8441733
GAGCGAC2350.037.0155752
AGCAGGT18950.036.3387768
CGAAGCG2201.6370905E-1136.2431459
AAAGCAG77050.035.934886
AGCAAAA62050.035.397452
Produced by FastQC (version 0.11.5)