FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n02_TR_562-3_v2_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n02_TR_562-3_v2_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682870
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG24860.14772382893509303No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC24450.14528751478129623No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG22940.13631474801975196No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA18350.10903991395651476No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT18200.10814857951000376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7100.069.424761
TATTCGC702.3382563E-762.1322361
GAGCAAA45850.049.9587481
GCGAAAG10450.049.9436152
GTTACGT3550.049.005711
CGAAAGC10700.048.775263
AGCGAAA11050.047.2317471
TTACGTG3900.046.4664152
TACGTGT4350.041.658313
GCGACTA1502.6058842E-838.6589134
CGGAATA1901.2914825E-1038.1513756
CAAAAGC61750.036.5068444
GGCCGTA1604.5996785E-836.2438052
GATATAC6250.035.9527853
CGTTGAC1458.716779E-734.992983
AAGCAGG78150.034.22647
GCAAAAG66050.034.1301733
CGAAGCG3000.033.828569
ACGTGTT5400.033.5580834
GTTATGT12550.033.500251
Produced by FastQC (version 0.11.5)