FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n02_TR_559-5_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n02_TR_559-5_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1394350
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA13000.091.4356161
GCGAAAG15050.078.496522
CGAAAGC15500.076.212123
CGACGAA1550.060.797947145
GAGCGAA5950.056.034531
GAAAGCA36850.032.6466564
CGCCGTG1000.006079419528.995943145
GAGCAAA29450.028.794891
CAAAAGC30050.027.2522324
GTATTAG6100.026.140131
AAAGCAG70200.024.6760525
GCAAAAG33200.024.2299793
ATACCGT1550.00181145123.381316
AGCAAAA35350.022.9629632
AGCGGTT1952.617936E-422.304571145
AAGCAGG80900.019.6212236
TAATACT12800.017.5517254
CTAACCG3905.636015E-616.7242243
GCAGGGG35000.016.567928
AGCAGGG68300.016.1307057
Produced by FastQC (version 0.11.5)