FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n02_TR_540-2_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n02_TR_540-2_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1784232
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA56200.060.7503931
GCGAAAG15800.058.265452
CGAAAGC16000.057.5371363
GAGCGAA13350.055.3838841
AGCGAAA17050.053.568641
CAAAAGC67000.049.7690934
GCAAAAG67500.049.721213
AGCAAAA75150.044.8526842
AAAGCAG99250.040.8998766
AAAAGCA94250.035.8390775
AAGCAGG112500.034.278647
GTTACGT1957.010385E-933.4558751
GTAACGG3108.62201E-1025.7214531
CGGTAAC1600.00217795522.6524161
GCAGGGG48650.022.6477589
CGGGTTG4058.094503E-1021.4772435
AGACGAC3055.3537224E-721.3898223
GTTATGT12350.019.9561351
TAACGGG4001.6369086E-819.9341262
CCGGTAC1850.0050533419.5912783
Produced by FastQC (version 0.11.5)