FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n02_TR_531-7_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n02_TR_531-7_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119457
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG42270.3775937798414767No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA30350.27111358453250106No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA18460.16490137629225599No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA17580.1570404222761571No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC17490.15623646106996517No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG16630.14855416509968672No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG15360.1372093791900895No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC14360.12827647689906801No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT14170.12657922546377395No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC13970.12479264500556966No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG13300.11880760047058528No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA12670.11317987202724177No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT12180.10880274990464125No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT12010.10728415651516762No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5850.0122.646931
AGCGAAA9650.073.609582
GCGAAAG10000.071.7612843
CGAAAGC10250.070.014144
GAGCAAA34550.066.4949341
GTTACGT1650.061.4923931
GCGATTA1200.060.410515145
GCAGGTA11450.059.5109379
GCGAAAT553.221496E-452.719554
AAGCAGG57150.052.5119937
AAACGCC701.6971371E-551.780445145
GATATAC3250.051.2978823
GCGACTA1004.7093636E-850.7425654
AGCAGGT18200.049.7866638
TGACGCC752.5523137E-548.32842145
CAATTCG3450.048.3262569
CCCGCTC3900.048.3240933
CTATAAC1700.046.9048964
CCGCTCT4050.046.5363924
GGCGAAA657.343869E-444.6068573
Produced by FastQC (version 0.11.5)