FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n02_TR_529-7_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n02_TR_529-7_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241718
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5400.087.2402041
GCGAAAG7900.058.7056543
CGAAAGC8000.057.9718364
AGCGAAA8100.057.2561342
GAGCAAA41200.055.4128381
CAAAAGC48550.045.0759354
CGAAGCG1307.381459E-944.5955165
AAGCAGG63150.041.5429467
GCAAAAG54350.040.2656253
AAAGCAG66400.039.837026
AGCAAAA57300.038.319082
GGTTATA3950.036.696951
GCGACTA1251.2695489E-534.78314
AGACGAC2051.1445081E-831.8138143
AAAAGCA70000.031.4716955
GATATAC3700.031.3361283
GCAGGGG32300.028.4969439
CGACTCC2405.2945325E-827.1764876
AGCAGGT14000.026.9198368
TATACTG7400.026.4409245
Produced by FastQC (version 0.11.5)