FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n02_TR_527-5_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n02_TR_527-5_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1415704
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG70380.4971378197702344No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA55220.39005328797545247No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA37860.26742878454818236No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT31270.22087950588541108No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA30340.21431033605895017No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC28900.20413871826313973No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT28230.19940609053870018No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT27260.19255437577346676No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA25730.18174703186541818No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT25680.18139385069195257No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT25220.17814458389606866No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC23730.1676197849267926No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC23600.16670151387578194No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC22990.16239270355950114No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT21360.15087899730452128No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG21350.15080836106982815No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT21320.15059645236574876No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG20360.14381537383520848No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT20280.14325028395766348No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC18240.12884049208026538No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA17620.12446104552929144No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG17450.12326022953950824No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC17290.12213004978441822No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC17150.12114114249871441No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA17120.12092923379463505No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA16620.11739742205997865No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC16540.11683233218243362No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT16120.11386561032532225No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC15930.11252352186615282No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG15900.11231161316207343No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG15280.1079321666110995No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC15250.10772025790702011No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC15090.10659007815193007No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT15030.10616626074377129No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT15010.10602498827438503No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT14840.10482417228460186No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT14680.10369399252951182No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC14580.10298763018258053No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT14540.10270508524380803No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT14400.10171617795810424No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC14360.10143363301933171No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAAGTC100.007097269144.958314
GAGCGAA3000.0132.854971
TGACGCC403.274181E-11126.86993145
CGTAACC203.8670067E-4108.74565145
CGGCGGG352.7630085E-7103.56729145
AGCGAAA8350.090.257481
GCAGGTA13400.086.0105069
CCTCGCC353.4384517E-582.85383145
GCGAAAG10100.076.056472
GTAGAAA10100.074.618811
CGAAAGC10300.074.579653
AGGCTCC2250.074.10815145
TCACACC754.8585207E-967.66396145
GCAGGGT23250.065.783769
GCTCTCC350.003567373662.140373145
GAGCAAA48500.055.881261
TCACGCC400.00605235954.372826145
GACAGGT400.006057441754.361297
TAGAAAC14150.053.7755132
AAGCAGG59150.053.1818587
Produced by FastQC (version 0.11.5)