FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n02_TR_526-2_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n02_TR_526-2_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377169
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA41100.073.8959051
GAGCGAA10750.067.427911
GCGAAAG13650.055.7536432
CAAAAGC54300.055.131324
GCAAAAG55250.054.0521663
AGCGAAA14400.053.3570181
AGCAAAA56450.052.8992962
CGAAAGC15450.048.7924923
AAAGCAG84800.043.6822286
AAAAGCA70550.042.4342965
AAGCAGG97550.036.1894077
TAAGCGC1050.00771448927.6153416
GTATAAG4900.025.1478581
GTTACGT2351.3334466E-624.6757471
GCAGGGG43950.024.2458959
GTGATCG1550.001810244823.383969
TTATCGC1600.002179099222.649922
TTACGTG2755.225651E-621.0850122
TATAGAC5900.020.8839912
GTATTAT18650.020.5989571
Produced by FastQC (version 0.11.5)