FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n02_TR_420-5_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n02_TR_420-5_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences939285
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA15510.16512560085597022No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC14170.15085943031135385No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG13160.14010657042324748No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG11980.12754382322724198No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA11740.12498868820432563No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG10430.1110419095375738No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2950.0103.197731
ATACCGG502.310595E-672.47646
CGAAAGC4400.067.534834
GATACCG554.069265E-665.8876345
CGATACC554.069265E-665.8876344
AGCGAAA4700.063.224092
GTCGCCC1300.061.335987145
TACCGGA606.81842E-660.3977
TACGTGT1450.059.9836653
GTTACGT1500.057.9872971
GGGTTAC902.0529114E-856.3765371
TTACGTG1550.056.1107642
GCGAAAG5950.056.0351563
CGACGAA805.880029E-754.365993145
GTCACGT1550.051.4403461
GTCCGAT858.9377136E-751.165261
GAGCAAA26150.048.507541
CCGATAC803.7404865E-545.3001633
TCCGATA803.7416637E-545.2977522
GCATCGT1652.910383E-1143.9297681
Produced by FastQC (version 0.11.5)