FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n02_TR_199-5_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n02_TR_199-5_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916756
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG24330.12693321424323178No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC22500.11738583314725504No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG21930.11441205870752458No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA21760.11352514352374533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6700.098.448661
GTCGAGA903.6379788E-1272.480395
AGCGAAA12300.066.0018541
CGAAAGC13150.062.2819023
GAGCAAA59500.060.545611
GCGAAAG13700.060.312152
TATTCGC450.00964878848.3227841
GATATAC5350.047.415843
GGTTATA4450.047.236881
GTTACGT3750.046.3898741
TTACGTG4150.043.6628882
CAAAAGC83350.042.8730664
AAGCAGG99800.042.2659157
TACGTGT4350.041.654313
GCAAAAG86350.041.044573
AGCAAAA86300.040.9014472
TCGAGAG1601.0149961E-940.7680936
AAAGCAG105350.039.970496
TAGTCGG2600.036.2401968
TATACTG8750.035.6189355
Produced by FastQC (version 0.11.5)