FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n02_TR_199-2_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n02_TR_199-2_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590975
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG40140.25229811907792393No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA26860.16882729144078318No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA22410.14085702163767502No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC18780.11804082402300477No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT16130.10138437121890663No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC16070.10100724398560632No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4550.0103.550551
TACGTGT1650.065.887513
GTTACGT1600.063.4247131
AGCGAAA8400.062.9932561
CGAAAGC8500.062.2443243
GCAGGTA13100.057.5456549
GCGAAAG9950.053.9036452
GCGACTA1101.6807462E-952.7149854
TTACGTG2000.050.734982
GAGCAAA48900.050.69531
TGACGCC1152.4883775E-950.42779145
AGTCGGC2250.048.3220755
AAGCAGG62250.047.0412457
GATATAC2950.046.679633
CGAAGCG1402.728484E-1046.597759
CAATTCG2500.046.3906489
GAGCGAC1601.0149961E-940.769182
GTAGAAA12100.040.735591
GGTTATA2150.040.456961
GCAAAAG61100.040.3304943
Produced by FastQC (version 0.11.5)