FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n02_TR_196-3_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n02_TR_196-3_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543439
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC18260.11830723468825137No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG18100.11727058860116919No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA17500.1133831657746111No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG16900.10949574294805303No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT15720.10185047805582209No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4500.088.584811
ATTACGC350.00357198462.1203883
AGACGAC3050.054.652363
TTACGTG2850.053.3982732
GTTACGT3000.050.734941
GAGCAAA45600.050.385261
AGCGAAA7800.050.1774141
CGAAAGC8150.048.9118584
TACGTGT3200.047.560923
GCGAAAG8750.047.2114943
ACTCCGA2350.043.1786771
AAGCAGG61900.042.9732937
CGACTCC3950.042.204036
TATGCGT700.001058194941.4176186
TGAAGCG2350.040.095785
CAATTCG2200.039.5359
CGAAGCG2057.2759576E-1238.8921555
CAAAAGC61250.037.274654
GACGACT4500.037.044564
AAAGCAG73050.036.513286
Produced by FastQC (version 0.11.5)