FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n02_TR_179-7_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n02_TR_179-7_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568999
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG38960.24831118439208694No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA26970.17189303498600061No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC23060.14697268768176397No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA21830.13913329454002202No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT19390.12358197806372087No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC19290.12294462902780691No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC18460.11765463202972086No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT17970.11453162175374236No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT17090.10892295023769932No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT16430.10471644660066705No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4800.089.090571
AGCGAAA9450.066.728041
CGAAAGC10300.062.6227573
GCGAAAG10400.062.0225942
GCAGGTA10800.056.36839
GAGCAAA49150.048.3694651
ACTCCGA1255.2114046E-946.3874931
TGACGCC1602.0008883E-1145.30029145
GCAAAAG55350.043.0782173
CAAAAGC55750.042.9004974
GTAGAAA12150.041.7582931
AAGCAGG68450.040.867637
TCCGAGG1807.2759576E-1140.2630733
GTATAGC4000.039.8642541
AGCAAAA62650.037.9442562
AAAGCAG74750.037.4232646
GCGACTA800.00204030236.237924
CATACTC7500.034.7872923
CTACACT9750.034.193734
GTCATAC8500.034.1084561
Produced by FastQC (version 0.11.5)