FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n02_TR_156-5_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n02_TR_156-5_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5131081
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG326650.6366104920191282No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA227550.44347380210914616No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA127120.24774506580582142No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC126220.24599104944942404No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT114520.22318883681625765No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC113820.22182460187239297No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT95660.1864324496144185No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG94050.18329470924352978No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT92380.18004003444888125No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC88630.17273163296389202No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT86430.16844403742603167No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG85430.16649513036336788No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC84070.1638446167581451No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT82380.1605509638222433No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT80380.1566531496969157No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA75250.14665525646545047No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA74770.14571978107537184No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT74210.1446283931202801No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT71140.13864524843790227No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT71100.1385672921553957No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC69560.13556597527889347No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA69070.13461101081818821No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG66100.12882275684207675No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT65860.12835501914703742No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC65430.12751698911009202No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG64720.12613326509560072No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG64470.12564603832993476No Hit
GAATCTCCTTTTTTAACTAGCCATATTAAATTTTTGTAGAAGCTTTTTGC61990.12081274881452855No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC58150.11332894569389959No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG56970.11102923535995632No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC56030.10919726272105235No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG55000.10718988844650865No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA54860.10691704145773571No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG53040.1033700306036876No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC52530.10237608800172907No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC52080.10149907982353037No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC51460.1002907574446788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA14050.0129.994021
AGCGAAA35700.087.0939941
GCAGGTA50150.082.0970159
AGGCTCC9150.079.22278145
GCGAAAG39550.078.982332
CGAAAGC40350.077.957533
CCGCACC1800.072.48884145
GCAGGGT105500.071.042479
GTAGAAA38450.067.8586651
TCACGCC1750.066.27551145
TTATAGG7450.065.1805041
GAGCAAA214000.061.4359631
GCAAAAG223050.058.782523
AGTAGAA25800.058.7118451
CCTCGCC2450.056.21584145
GACAGTA5350.055.547327
AGCAAAA239950.054.5803832
AAGCAGG274300.054.51387
ATACCGG400.00605996454.3576161
CAAAAGC244300.053.5205654
Produced by FastQC (version 0.11.5)