FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n02_TR_122-7_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n02_TR_122-7_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981902
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG12840.13076661418349284No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA12170.12394312263341963No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA11970.12190625948414403No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG11940.1216007300117527No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC11430.11640672898109994No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG10900.11100904163551964No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG10650.10846296269892515No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT9920.10102841220406925No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2750.0110.677541
CGAAAGC4500.069.253724
AGCGAAA4750.065.598762
GCGAAAG5650.061.5621223
TTACGTG1457.2759576E-1249.9749952
GTTACGT1600.049.821361
AAGCAGG34350.049.376537
GAGCAAA28900.048.8967131
GGGTTAC1501.0913936E-1148.3116261
ACTCCGA1109.9942554E-846.1156431
GTCGAAG1453.8744474E-1044.9774973
CAATTCG1652.910383E-1143.928619
TACGTGT1652.910383E-1143.9174233
GTAATGC1505.3842086E-1043.4804651
AGCAGGT10250.040.307188
ATACGGA907.497669E-540.263794
GATATAC2900.039.9799963
TATGTGT6550.039.8274043
TATAGGT3300.039.5317234
TCTCGGG951.0297457E-438.15242145
Produced by FastQC (version 0.11.5)